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《医学前沿(英文)》 2018年 第12卷 第2期 页码 196-205 doi: 10.1007/s11684-017-0560-5
We employed a multiplex polymerase chain reaction (PCR) coupled with capillary electrophoresis (mPCR-CE) targeting six Clostridium difficile genes, including tpi, tcdA, tcdB, cdtA, cdtB, and a deletion in tcdC for simultaneous detection and characterization of toxigenic C. difficile directly from fecal specimens. The mPCR-CE had a limit of detection of 10 colony-forming units per reaction with no cross-reactions with other related bacterial genes. Clinical validation was performed on 354 consecutively collected stool specimens from patients with suspected C. difficile infection and 45 isolates. The results were compared with a reference standard combined with BD MAX Cdiff, real-time cell analysis assay (RTCA), and mPCR-CE. The toxigenic C. difficile species were detected in 36 isolates and 45 stool specimens by the mPCR-CE, which provided a positive rate of 20.3% (81/399). The mPCR-CE had a specificity of 97.2% and a sensitivity of 96.0%, which was higher than RTCA (x2 = 5.67, P = 0.017) but lower than BD MAX Cdiff (P = 0.245). Among the 45 strains, 44 (97.8%) were determined as non-ribotype 027 by the mPCR-CE, which was fully agreed with PCR ribotyping. Even though ribotypes 017 (n = 8, 17.8%), 001 (n = 6, 13.3%), and 012 (n = 7, 15.6%) were predominant in this region, ribotype 027 was an important genotype monitored routinely. The mPCR-CE provided an alternative diagnosis tool for the simultaneous detection of toxigenic C. difficile in stool and potentially differentiated between RT027 and non-RT027.
关键词: Clostridium difficile multiplex PCR capillary electrophoresis detection characterization
LI Yongfeng, REN Nanqi, LI Jianzheng, LI Peng, YANG Chuanping
《能源前沿(英文)》 2007年 第1卷 第4期 页码 403-407 doi: 10.1007/s11708-007-0058-2
关键词: fermented biohydrogen high important identification biohydrogen
High production of butyric acid by
Chao Ma,Jianfa Ou,Matthew Miller,Sarah McFann,Xiaoguang (Margaret) Liu
《化学科学与工程前沿(英文)》 2015年 第9卷 第3期 页码 369-375 doi: 10.1007/s11705-015-1525-3
关键词: Clostridium tyrobutyricum butyric acid production fermentation mutant pH flux balance analysis
标题 作者 时间 类型 操作
Simultaneous detection and characterization of toxigenic:Clostridium difficile directly from clinical
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期刊论文
Molecular characterization and fermentative hydrogen production of a wild anaerobe in clostridium genus
LI Yongfeng, REN Nanqi, LI Jianzheng, LI Peng, YANG Chuanping
期刊论文